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murine macrophage tlr knockout cell lines δmyd88  (BEI Resources)

 
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    BEI Resources murine macrophage tlr knockout cell lines δmyd88
    The innate immune receptor <t>TLR2</t> and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.
    Murine Macrophage Tlr Knockout Cell Lines δmyd88, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine macrophage tlr knockout cell lines δmyd88/product/BEI Resources
    Average 90 stars, based on 1 article reviews
    murine macrophage tlr knockout cell lines δmyd88 - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response"

    Article Title: Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2025.1517822

    The innate immune receptor TLR2 and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.
    Figure Legend Snippet: The innate immune receptor TLR2 and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.

    Techniques Used: Knock-Out, Incubation, Infection, Expressing, Enzyme-linked Immunosorbent Assay, Purification, Sandwich ELISA

    Mtb PE18 and PPE26 employ TLR2 and Myd88 for regulating immune response and endosome-phagosome maturation: A Model. Mtb PE18 and PPE26 heterodimers interact with the innate immune receptor TLR2, recruiting adapter Myd88 to initiating downstream signaling cascades and the consequent secretion of proinflammatory cytokines and NO. PE18 and PPE26 primed peritoneal macrophages and splenocytes showed increased expression of activation markers, proinflammatory cytokine production, and skewed memory response towards T-cells cell-mediated effector memory. PE18 and PPE26 proteins inhibit actin-mediated cytoskeleton dynamics by regulating the protein levels of Arp2, N-Wasp, Profilin, and Rac1. In addition to the regulation of actin dynamics, PE18 and PPE26 possibly hampers endosome-phagosome maturation by modulating the early, late, and recycling endosome markers EEA1, Rab5, Rab7, and Rab11. These findings highlight that Mtb PE18 and PPE26 mediated subversion of innate defenses leads to efficient survival and virulence.
    Figure Legend Snippet: Mtb PE18 and PPE26 employ TLR2 and Myd88 for regulating immune response and endosome-phagosome maturation: A Model. Mtb PE18 and PPE26 heterodimers interact with the innate immune receptor TLR2, recruiting adapter Myd88 to initiating downstream signaling cascades and the consequent secretion of proinflammatory cytokines and NO. PE18 and PPE26 primed peritoneal macrophages and splenocytes showed increased expression of activation markers, proinflammatory cytokine production, and skewed memory response towards T-cells cell-mediated effector memory. PE18 and PPE26 proteins inhibit actin-mediated cytoskeleton dynamics by regulating the protein levels of Arp2, N-Wasp, Profilin, and Rac1. In addition to the regulation of actin dynamics, PE18 and PPE26 possibly hampers endosome-phagosome maturation by modulating the early, late, and recycling endosome markers EEA1, Rab5, Rab7, and Rab11. These findings highlight that Mtb PE18 and PPE26 mediated subversion of innate defenses leads to efficient survival and virulence.

    Techniques Used: Expressing, Activation Assay



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    murine macrophage tlr knockout cell lines δmyd88  (BEI Resources)
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    BEI Resources murine macrophage tlr knockout cell lines δmyd88
    The innate immune receptor <t>TLR2</t> and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.
    Murine Macrophage Tlr Knockout Cell Lines δmyd88, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/murine macrophage tlr knockout cell lines δmyd88/product/BEI Resources
    Average 90 stars, based on 1 article reviews
    murine macrophage tlr knockout cell lines δmyd88 - by Bioz Stars, 2026-03
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    tlr knockout cell lines  (BEI Resources)
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    The innate immune receptor <t>TLR2</t> and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.
    Tlr Knockout Cell Lines, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The innate immune receptor TLR2 and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.

    Journal: Frontiers in Immunology

    Article Title: Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response

    doi: 10.3389/fimmu.2025.1517822

    Figure Lengend Snippet: The innate immune receptor TLR2 and adaptor Myd88 are involved in the secretion of proinflammatory cytokines. (A-C) 6-well tissue culture plates were used for seeding 2 million TLR knockout macrophage cells. The cells were incubated overnight for adherence. After adherence, cells were infected with Msmeg , Msmeg_VC , and Msmeg expressing PE18, PPE26, and PE18+PPE26 (MOI = 1:10). Culture supernatants were collected after 24 and 48 h of infection. The levels of TNFα, IL6, and IL12 were quantified using ELISA. (D-F) 6-well culture plates were used for seeding ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88 mouse macrophages (2 million cells/well) and allowed to adhere for 2 h. PE18 (5 µg), PPE26 (5 µg), and PE18 (2. 5µg) + PPE26 (2.5 µg) purified proteins were used to treat macrophage cells for 24 h. The culture supernatant was collected, and TNFα, IL6, and IL12 levels were measured using sandwich ELISA. Untreated (UT) cells, Proteinase K-digested, and heat-inactivated protein (HI) (5 µg)-treated cells were used as negative controls. Three independent experiments were performed to determine the level of pro-inflammatory cytokines. ****P values less than 0.0001 as compared to the controls. n. s shows that the data were not significant.

    Article Snippet: The Biodefense and Emerging Infections Research (BEI) resources (NIAID, NIH) provided the murine macrophage TLR knockout cell lines ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88.

    Techniques: Knock-Out, Incubation, Infection, Expressing, Enzyme-linked Immunosorbent Assay, Purification, Sandwich ELISA

    Mtb PE18 and PPE26 employ TLR2 and Myd88 for regulating immune response and endosome-phagosome maturation: A Model. Mtb PE18 and PPE26 heterodimers interact with the innate immune receptor TLR2, recruiting adapter Myd88 to initiating downstream signaling cascades and the consequent secretion of proinflammatory cytokines and NO. PE18 and PPE26 primed peritoneal macrophages and splenocytes showed increased expression of activation markers, proinflammatory cytokine production, and skewed memory response towards T-cells cell-mediated effector memory. PE18 and PPE26 proteins inhibit actin-mediated cytoskeleton dynamics by regulating the protein levels of Arp2, N-Wasp, Profilin, and Rac1. In addition to the regulation of actin dynamics, PE18 and PPE26 possibly hampers endosome-phagosome maturation by modulating the early, late, and recycling endosome markers EEA1, Rab5, Rab7, and Rab11. These findings highlight that Mtb PE18 and PPE26 mediated subversion of innate defenses leads to efficient survival and virulence.

    Journal: Frontiers in Immunology

    Article Title: Deciphering the functional roles of PE18 and PPE26 proteins in modulating Mycobacterium tuberculosis pathogenesis and immune response

    doi: 10.3389/fimmu.2025.1517822

    Figure Lengend Snippet: Mtb PE18 and PPE26 employ TLR2 and Myd88 for regulating immune response and endosome-phagosome maturation: A Model. Mtb PE18 and PPE26 heterodimers interact with the innate immune receptor TLR2, recruiting adapter Myd88 to initiating downstream signaling cascades and the consequent secretion of proinflammatory cytokines and NO. PE18 and PPE26 primed peritoneal macrophages and splenocytes showed increased expression of activation markers, proinflammatory cytokine production, and skewed memory response towards T-cells cell-mediated effector memory. PE18 and PPE26 proteins inhibit actin-mediated cytoskeleton dynamics by regulating the protein levels of Arp2, N-Wasp, Profilin, and Rac1. In addition to the regulation of actin dynamics, PE18 and PPE26 possibly hampers endosome-phagosome maturation by modulating the early, late, and recycling endosome markers EEA1, Rab5, Rab7, and Rab11. These findings highlight that Mtb PE18 and PPE26 mediated subversion of innate defenses leads to efficient survival and virulence.

    Article Snippet: The Biodefense and Emerging Infections Research (BEI) resources (NIAID, NIH) provided the murine macrophage TLR knockout cell lines ΔTLR2, ΔTLR4, ΔTRIF, and ΔMyd88.

    Techniques: Expressing, Activation Assay